MUTANT OF LAMBDA BACTERIOPHAGE PRODUCING A THERMOLABILE ENDOLYSIN

Mutant of Lambda Bacteriophage Producing a Thermolabile Endolysin.

Campbell, Allan (University of Rochester, Rochester, N.Y.) and Alice del Campillo-Campbell. Mutant of lambda bacteriophage producing a thermolabile endolysin. J. Bacteriol. 85:1202-1207. 1963.-Endolysin from lambda bacteriophage and a temperature-sensitive mutant thereof was partially purified. The mutant enzyme was distinguishable from the wild type by its greater rate of inactivation by high ...

A bacteriophage endolysin that eliminates intracellular streptococci

PlyC, a bacteriophage-encoded endolysin, lyses Streptococcus pyogenes (Spy) on contact. Here, we demonstrate that PlyC is a potent agent for controlling intracellular Spy that often underlies refractory infections. We show that the PlyC holoenzyme, mediated by its PlyCB subunit, crosses epithelial cell membranes and clears intracellular Spy in a dose-dependent manner. Quantitative studies using...

Genetic map of bacteriophage lambda.

Relation of endolysin to lysis by lambda bacteriophages.

the chambers. (ii) A drop of the culture is placed on the grid and then withdrawn until the fluid level is just below the surface of the grid; if the surface is wet, the covering film will not stick properly. (iii) The floor of the chamber is formed by dipping the cover slip through a film which has been previously cast on glass and stripped off onto a water surface; casting, stripping, and dip...

Construction of the Recombinant Plasmid Expressing AID under the Control of Temperature-sensitive Promoter of Bacteriophage Lambda

Background and purpose: Activation-induced cytidine deaminase (AID) is a B-cell specific enzyme responsible for somatic hypermutation (SHM) and class switch recombination (CSR) of antibody genes within the B-cell follicle of peripheral lymphoid organs. Ectopic overexpression of the enzyme leads to mutations in non-B cells and Escherichia coli (E.coli) genes. However, induction of mutations in E...